A first loss in a decade to their oldest rivals sees the world's second-ranked side give up their hopes of a Six Nations Grand Slam.
Six Nations: Scotland defeat the 'best lessons in the world' for England, insists Eddie Jones
England coach Eddie Jones is confident a stunning 25-13 defeat by Scotland will provide his Six Nations champions with the "best lessons in the world".
Scotland threw this season's title race wide open with their first win over old rivals England in a decade.
The first half saw Scotland score three tries - a tally made all the more remarkable given it was 14 years since they had last crossed England's try-line in a Calcutta Cup clash at Murrayfield.
Huw Jones went over for two tries and Sean Maitland also touched down, with impressive fly-half Finn Russell orchestrating all three scores before Scotland closed out the match.
This was just England's second defeat in 26 Tests under Eddie Jones, whose ultimate aim with is to win next year's World Cup.
But Saturday's defeat raised some major questions over England's ability to dethrone New Zealand in Japan, with Jones's men conceding a costly 13 penalties as they were over-run by a ferocious Scotland at the breakdown.
"These lessons you don't want to have but they are the best lessons in the world," said Eddie Jones. "We will learn a lot from this. Unfortunately the lesson isn't sometimes nice to take but it is a great lesson for us. We weren't there in the first half and I take responsibility for it.
"We are a team that are in a competition where everyone wants to beat us and every game is a challenge for us. It is not the end of the world -- the end of the world might come when the Beast From the East comes."
Reports emerged before kick-off of a fracas in the tunnel between England's Owen Farrell and Scotland's Ryan Wilson following the pre-match warm-ups.
Both camps played down the significance of the incident, with Jones saying: "I don't know about it. If there was, we shouldn't let that detract from a great Scotland victory. Don't get distracted by other things."
This was arguably Scotland's best performance since coach Gregor Townsend took charge last year.
Scotland played well in a 22-17 defeat by world champions New Zealand at Murrayfield in November, but they never looked like letting things slip against an England side who are second in the global rankings.
"It is the biggest win from a coaching perspective," Townsend said. "We came close to beating the No 1 team in the world and have beaten the No 2.
"Our defence was outstanding in both games but tonight we finished off our opportunities. It was not the complete performance, but the way we defended in the second half when the game was won and keeping them out was very satisfying."
Next up for Scotland is a match away to Ireland, the only team left in this season's Six Nations who can complete a Grand Slam.
It is eight years since Scotland won a Six Nations game on the road anywhere other than Rome -- a 23-20 victory against Ireland in Dublin.
But having ended one unwanted record against England, former Scotland playmaker Townsend challenged his side to snap another.
"We have issues away from home," he said. "We have to show a truer picture of what we are about when we are away."