Jose Mourinho's nine-year unbeaten home record in league matches comes to an end as Real Madrid suffer a shock 1-0 loss to Sporting Gijon in the Primera Liga.
Mourinho's unbeaten run ends as Sporting Gijon upset Real Madrid
Miguel de las Cuevas 79th minute winner also gives Barcelona, Real's league rivals, a chance to extend their five-point lead when they visit Villareal later tonight.
De Las Cuevas broke free on the edge of the area and fired in a right-footed shot off the post for the winner. The absence of Cristiano Ronaldo, Karim Benzema and Xabi Alonso showed as Real struggled to create chances in front of goal.
Gonzalo Higuain returned to the pitch for the first time since last November as a second-half substitute but could not spark his team's attack.
The Portuguese coach last experienced a league defeat in front of his home supporters when Porto lost to Beira Mar on February 23 2002.
In the time since, he remained unbeaten at Stamford Bridge, the home of Chelsea, and at the San Siro in his time in charge of Inter Milan before joining Real at the end of last season.
The defeat also spoiled Real's perfect home record in the Primera Liga this season. They had won all 14 of their previous matches at the Bernabeu.